Controlled release of recombinant human cementum protein 1 from electrospun multiphasic scaffold for cementum regeneration
15 Jul 2016
Periodontitis is known to affect about 15% of the adult population and is a major cause of tooth loss. Cementum regeneration is critical in overcoming this. In this study PEG-stabilized amorphous calcium phosphate nanoparticles were loaded with recombinant human CEMP1 (which can induce cementogenic differentiation). An electrospun multiphasic scaffold incorporating rhCEMP1/ACP was fabricated from poly(ε-caprolactone) and type I collagen. A critical size defect rat model was used to evaluate the scaffolds in vivo and demonstrated controlled release for up to 4 weeks and cementum-like tissue formation but little bone formation after 8 weeks. In vitro co-culture studies with the scaffold and human periodontal ligament cells, indicated upregulation of cementoblastic marker expression and downregulation of osteoblastic markers including osteocalcin and osteopontin after 7 days.